Transforming growth factor-β1 gene polymorphisms are associated with progression of liver fibrosis in Caucasians with chronic hepatitis C infection

Figure 2 LC analysis of the Leu10Pro polymorphism. A: The derivative melting curves of a representative LC analysis for different samples are plotted. Genotypes identified are: Leu10Leu (black line), Leu10Pro (red line), and Pro10Pro (green line). The different allelic variants of codon 10 show typical melting temperatures at T_m = 56 °C (Leu10) and T_m = 67 °C (Pro25), respectively. A no-template control is shown in blue; B: Sequencing was performed to confirm the determined genotypes; C: Representative LC products were separated in 2 g/L agarose gel to demonstrate the correct size of synthesized amplicons (523 bp). The sizes of selected molecular weight markers are indicated at the left margin.