Copyright
©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Apr 7, 2005; 11(13): 1929-1936
Published online Apr 7, 2005. doi: 10.3748/wjg.v11.i13.1929
Published online Apr 7, 2005. doi: 10.3748/wjg.v11.i13.1929
Table 2 LC Protocol 2 for Genotyping the TGF-β1 Gene Polymorphisms.
| Codon 25 (Arg25Pro) | ||||
| Time (s) | Temp (°C) | Transition Rate (°C/s) | Cycles | |
| 600 | 95 | 20 | 1 | Denaturation |
| 10 | 95 | 20 | 45 | Cycling |
| 10 | 59 | 20 | ||
| 20 | 72 | 20 | ||
| 60 | 95 | 20 | 1 | Melting curve |
| Analysis | ||||
| 30 | 40 | 20 | ||
| 0 | 80 | 0.2 | ||
| 5-d(CTAGGTTATTTCCGTGGG)-3’ | Sense primer | |||
| 5-d(CCTTGGCGTAGTAGTCG)-3’ | Reverse primer | |||
| 5-d(GCTACCGCTGCTGTGGCTACTGGTGCT-Fluo)-3 | Anchor primer | |||
| 5-LCRed640-(ACGCCTGGCCCGCCG-P)-3’ | Sensor primer | |||
- Citation: Wang H, Mengsteab S, Tag CG, Gao CF, Hellerbrand C, Lammert F, Gressner AM, Weiskirchen R. Transforming growth factor-β1 gene polymorphisms are associated with progression of liver fibrosis in Caucasians with chronic hepatitis C infection. World J Gastroenterol 2005; 11(13): 1929-1936
- URL: https://www.wjgnet.com/1007-9327/full/v11/i13/1929.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i13.1929