Synergistic effects of interferon-alpha in combination with chemoradiation on human pancreatic adenocarcinoma

Figure 4 Secretion of VEGF Cells were seeded out after four-day treatment in a density of 1×10⁵ living cells in 2 mL. Supernatants from treated and untreated tumor cells were collected after 24 h and stored at -80 °C. VEGF concentration was determined by ELISA (A). B) shows the influence of IFN-α in combination treatments. Values of treatments without IFN-α were subtracted from values after combination treatment. Data from three cell lines are shown as mean±standard error from at least three separate experiments. ^aP<0.05 was labeled with an asterisk and ^bP<0.01 with two asterisks.