Synergistic effects of interferon-alpha in combination with chemoradiation on human pancreatic adenocarcinoma

Figure 2 Induction of apoptosis Apoptosis was determined after five-day treatment by staining for AnnexinV⁺ using flow cytometry (A). Late apoptosis is defined as AnnexinV⁺/PI⁺, necrosis as PI⁺. B) shows the influence of IFN-α in combination treatments. Values of treatments without IFN-α were subtracted from values after combination treatment. Data is shown as mean±SD error from eight cell lines with at least three separate experiments. ^aP<0.05 was labeled with an asterisk; ^bP<0.01 with two asterisks and ^dP<0.001 with three asterisks.