Sirolimus inhibits growth of human hepatoma cells alone or combined with tacrolimus, while tacrolimus promotes cell growth

Figure 2 Cell-cycle analysis of both cell lines after treatment for 24 h with sirolimus, tacrolimus, or the combination of both at a dose of 25 ng/mL each compound. M1 = Sub-G1 region, indicating cells with small DNA fragments, a typical feature of apoptosis; M2 = G1-Phase; M3 = S-Phase; M4 = G2/M-Phase. The different cell cycle phases (M1-M4) were set in a reference analysis and kept constant throughout the measurements.