Liver Cancer
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Mar 14, 2005; 11(10): 1411-1419
Published online Mar 14, 2005. doi: 10.3748/wjg.v11.i10.1411
Table 1 Primer sequence and expected lengths of fragments in RT-PCR analysis of rat liver selected genes and β-actin.
GenePrimer sequenceExpected lengths of fragments
β-actinForward5- GTCTTCCCCTCCATCGTG-3992 bp
Reverse5-TGCTTGCTGATCCACATCTG-3’
FGFR2Forward5-GGACAGACCCAAGGAGGCAG-3667 bp
Reverse5-GCCAGCAGTCCCTCATCATC-3’
FGFR4Forward5-GGAGGTGCTGTATCTGAGGAACGTG-3599 bp
Reverse5-TGTCGGAGGCATTGTCTTTCAG-3’
TGFβ R1Forward5-CGTCGCTGCCTGCTTCTCATC-3646 bp
Reverse5-CCGCCATTTGCCTCGCC-3’
TGFβ R2Forward5-CGACAACTGCGCCATCATCC-3649 bp
Reverse5-GGCCATGTATCTCGCTGTTCCC-3’
TGFβ R3Forward5-GGTGTGGCATCTGAAGACGGAG-3787 bp
Reverse5-GCTCAGGAGGAATGGTGTGGACT-3’
Collagen1Forward5- GCGAAGGCAACAGTCGATTC-3’69 bp
Reverse5-CCCAAGTTCCGGTGTGACTC-3’
Collagen3Forward5-CAGCTGGCCTTCCTCAGACTT-3’70 bp
Reverse5-GCTGTTTTTGCAGTGGTATGTAATG-3’
Abcb1Forward5-GGACCCACAGGCGGAGG-3634 bp
Reverse5-GCAGGGTTGTTGTAGGGCTCA-3’