Stable transfection of extrinsic Smac gene enhances apoptosis-inducing effects of chemotherapeutic drugs on gastric cancer cells

Figure 1 Cellular Smac expression detected by RT-PCR and Western blotting. A: Cellular Smac mRNA expression detected by RT-PCR. Lane 1: MKN-45 cells; lane 2: MKN-45/neo cells; lane 3: MKN-45/Smac cells; lane 4: PCR marker (100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, 700 bp, 800 bp, 900 bp, 1000 bp). B: Cellular Smac protein expression detected by Western blotting. Lane 1: MKN-45/Smac cells; lane 2: MKN-45/neo cells; lane 3: MKN-45 cells.