Differential gene expression in human hepatocellular carcinoma Hep3B cells induced by apoptosis-related gene BNIPL-2

Figure 3 Parallel analysis of gene expression profiles in Hep3B-Tet-on-BNIPL-2/13 cells before or after BNIPL-2 overexpression. Total RNA samples were purified from 1×10⁷ cells using TRIzol reagent before (A) or after (B) BNIPL-2 overexpression. They were reverse-transcribed into cDNA, labeled with [α-³²P] dATP, and hybridized to Atlas human cDNA expression array (#7740-1) according to the manufacturer’s protocol. Notes: The genes in the row G are housekeeping genes. Arrows indicate the three examples of differentially expressed genes (A6b, p120 antigen; C5n, Rad; D6f, p21).