Association of low p16INK4a and p15INK4b mRNAs expression with their CpG islands methylation with human hepatocellular carcinogenesis

Figure 3 Methylation analysis of p15 gene in human primary heparocarcinoma. MSP product of p15 gene from HCC tissues was electrophoresed on a 2.5% agarose gel. M: pBR322/HeaIII DNA molecular marker (in graph m) or PBR322/Msp I DNA marker (in graph u); N: Normal liver tissue DNA; HS: HS-Sul-tan DNA (positive control); C, P, N represent RNA from cancerous, paracancerous, non-cancerous liver tissue respectively; 1, 2 mark the HCC patient number; m: PCR products from methylation specific primers, u: PCR products from unmethylation specific primers.