Association of low p16INK4a and p15INK4b mRNAs expression with their CpG islands methylation with human hepatocellular carcinogenesis

Figure 2 Methylation analysis of p16 gene in human primary heparocarcinoma. MSP product of p16 gene from HCC tissues was electrophoresed on a 25 g/L agarose gel. M: pBR322/ HeaIII DNA molecular marker; N: Normal liver tissue DNA; HS: HS-Sultan DNA (positive control); C, P, N represent RNA from cancerous, para-cancerous, non-cancerous liver tissue respectively; 1, 2 mark the HCC patient number; m: PCR prod-ucts from methylation specific primers, u: PCR products from unmethylation specific primers.