Modulation of Kupffer cells on hepatic drug metabolism

Table 1 Influence of different dose of GdCl₃ on P450 content and P450 isoform activities

Group	P-450 (nmol/mg·pro)	NADPH-C (nmol/min·mg·pro)	ANH (nmol/min·mg·pro)	AMD (nmol/min·mg·pro)	EMD (nmol/min·mg·pro)
Control	1.4 ± 0.4	14.5 ± 2.8	0.038 ± 0.009	0.96 ± 0.07	0.89 ± 0.06
GdCl310-1 d	1.13 ± 0.25	10.9 ± 1.0b	0.032 ± 0.016	0.76 ± 0.07b	0.78 ± 0.12
GdCl320-1 d	0.87 ± 0.28b	10.5 ± 1.8b	0.033 ± 0.006	0.82 ± 0.03a	0.74 ± 0.13a
GdCl340-1 d	1.01 ± 0.29a	6.0 ± 1.0b	0.036 ± 0.010	0.77 ± 0.17a	0.74 ± 0.18a

GdCl₃10-1 d, GdCl₃20-1 d, GdCl₃40-1 d: Mice were intraperitoneally injected with GdCl₃ 10, 20, 40 mg/kg, respectively, and sacrificed after 1 d. The hepatic microsome was prepared to assess the content of P450 and the activities of P450 isoforms. P450: cytochrome P450; NADPH-C: NADPH-cytochrome c reductase; ANH: aniline hydroxylase; AMD: aminopytine N-demethylase; EMD: erythomycin N-demethylase (n = 8, mean ± SD,

^aP < 0.05,

^bP < 0.01 compared with control group).