Differential gene expression in human hepatocellular carcinoma Hep3B cells induced by apoptosis-related gene BNIPL-2

Table 1 Gene specific primers for PCR

Gene	GenBank accession number	Orientation	Sequence
BNIPL-2	AY033000	Forward	5’ CGGGATCCACCATGTACCCATACGATGTTCCAGATTACGCTCTTGGAACTATACAAGAG 3’
		Reverse	5’ CCATCGATCTATGTCCCTCCTGAGCCATGGAGATCCCGGTCCAGCTGTCTGACAGC 3’
β-actin	NM_001101	Forward	5’ AAGTACTCCGTGTGGATCGG 3’
		Reverse	5’ TCAAGTTGGGGGACAAAAAG 3’
Rad	L24564	Forward	5’ GCTGCCCTGCGAAGTGGCTC 3’
		Reverse	5’ TCCCTCCAGGGCAGGCACAC 3’
CLK-1	L29222	Forward	5’ GGGTGGTCCCAACCATGTGA 3’
		Reverse	5’ CCGGCAGAACTGTGTTCATCCCA 3’
p38 MAP kinase	L35253	Forward	5’ GTTGGAACCCCAGGGGCTGA 3’
		Reverse	5’ GGCATGTGCAAGGGCTTGGG 3’
p21	U09579	Forward	5’ CTGTGGGGGTGAGGGTCCCA 3’
		Reverse	5’ TGGGGAGGGATGGGGTGGAT 3’

Underlined sequences are restriction endonuclease recognition sites (italic) and HA-tag (bold).