Correlation of expression of multidrug resistance protein and messenger RNA with 99mTc-methoxyisobutyl isonitrile (MIBI) imaging in patients with hepatocellular carcinoma

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May 1, 2004 (publication date) through Dec 22, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. May 1, 2004; 10(9): 1281-1285
Published online May 1, 2004. doi: 10.3748/wjg.v10.i9.1281

Table 2 Oligonucleotides used for PCR amplifications

Gene	Quantification method	Sequence	cDNA
MDR1	Forward primer	5’- CCCAGGAGCCCATCCTGT-3’	3774-3791
	Revease primer	5’- CCCGGCTGTTGTCTCCATA-3’	3838-3821
	Probe	5’-(FAM)TGACTGCAGCATTGCTGAGAACATTGC(TAMRA)-3’3793-3819
MRP	Forward primer	5’- AAGCGCCTCGAGTCGGT-3’	3617-3633
	Revease primer	5’- TCGAATGACGCTGACCCC-3’	3694-3677
	Probe	5’-(FAM)AGCCGCTCCCCGGTCTATTCCC-(TAMRA)-3’	3635-3656
LRP	Forward primer	5’- TTTCGATGACTTCCATAAGAACTCA-3’	1881-1905
	Reverse primer	5’- TTCCGAGGTCTCAAAGCCAA-3’	1950-1931
	Probe	5’(FAM)-CCCGCATCATTCGCACTGCTGT- (TAMRA)3’	1907-1928
GAPDH	Forward primer	5’- GAAGGTGAAGGTCGGAGTCA-3’
	Revease primer	5’- GAAGATGGTGATGGGA-3’
	Probe	5’-(JOE)CAAGCTTCCCGTTCTCAGCC(TAMRA)-3’

Citation: Wang H, Chen XP, Qiu FZ. Correlation of expression of multidrug resistance protein and messenger RNA with ^99mTc-methoxyisobutyl isonitrile (MIBI) imaging in patients with hepatocellular carcinoma. World J Gastroenterol 2004; 10(9): 1281-1285
URL: https://www.wjgnet.com/1007-9327/full/v10/i9/1281.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i9.1281