The protective mechanism of Yisheng Injection against hepatic ischemia reperfusion injury in mice

Figure 7 RT-PCR analysis of TNF-α (A) and ICAM-1 (B) mRNA expression in the liver tissues. β-actin was used as control. Mice were subjected to 90 min of ischemia and 6 h of reperfusion. Saline or YS with different dose (5, 10, 20 mg/kg) was injected intraperitoneally 24 h and 1 h before ischemia and a third dose was injected intravenously just before reperfusion. Liver RNA extracts were analyzed by reverse transcription PCR and stained with SYBR green dye. M: marker; Lain 1: sham control; Lain 2: I/R group; Lain 3-5: 5, 10 and 20 mg/kg of YS treated groups. The band intensities of TNF-α (C) and ICAM-1 (D) in sham, I/R and YS-treated groups were compared. One representative experiment from three performed is shown. ^bP < 0.01 compared with sham group; ^cP < 0.05, ^dP < 0.01 compared with I/R group.