Adeno-associated virus mediated endostatin gene therapy in combination with topoisomerase inhibitor effectively controls liver tumor in mouse model

Figure 2 In vitro biological activities of expressed endostatin. A: 5 × 10³ HUVECs in a 96-well were cultured in the conditioned media from Hepa1c1c7 mouse hepatoma cells without pre-treatment (Z), those from the cells pretreated with etoposide (Z/et) or camptothecin (Z/cpt), those from the rAAV-endostatin transduced cells without pretreatment (E), those from rAAV-endostatin transduced cells pretreated with etoposide (E/et) or camptothecin (E/cpt). The number of cells was then calculated by a MTT assay. Each value represents mean ± SD of 3 independent experiments (P < 0.05, rAAV-endostatin in combination with pretreatment groups versus other groups). B: Impact on tube formation of endothelial cells. HUVECs were seeded into 24-well plates coated with Matrigel at a density of 5 × 10⁴ cells in each well and cultured in the conditioned media. After 18 h incubation, the level of cell growth and differen-tiation was observed. All tests were performed in triplicate.