Adeno-associated virus mediated endostatin gene therapy in combination with topoisomerase inhibitor effectively controls liver tumor in mouse model

Figure 1 Effects of topoisomerase inhibitors on rAAV mediated endostatin expression level. Hepa1c1c7 mouse hepatoma cells were transduced with 1 × 10⁴ particles/cell of rAAV-endostatin or rAAV-LacZ. In pretreatment group, etoposide (3 µmol/L) or camptothecin (10 µmol/L) was administered 6 h before transduction. Fourty-eight hours later, the expression of endostatin was determined. A: Analysis of protein expression by NuPAGE electrophoresis, B: Concentration of endostatin measured by ELISA (P < 0.05, rAAV-endostatin in combination with pretreatment groups versus other groups). Z: rAAV-LacZ without pretreatment, E: rAAV-endostatin without pretreatment, E/et: rAAV-endostatin pretreated with etoposide, E/cpt: rAAV-endostatin pretreated with camptothecin.