Construction of prokaryotic expression system of 2 148-bp fragment from cagA gene and detection of cagA gene, CagA protein in Helicobacter pylori isolates and its antibody in sera of patients

doi:10.3748/wjg.v10.i8.1183

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Apr 15, 2004 (publication date) through Jul 28, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 15, 2004; 10(8): 1183-1190
Published online Apr 15, 2004. doi: 10.3748/wjg.v10.i8.1183

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Figure 4 rCagA1 expression by pET32a-cagA1-E. coli BL21DE3 induced with IPTG. Lane 1: Protein marker; Lane 2: Blank control; Lane 3: Non-induced; Lanes 4-6: induced with 0.1, 0.5 and 1.0 mmol/L IPTG, respectively; Lanes 7 and 8: Bacterial supernatant and precipitate induced with 0.5 mmol/L IPTG, respectively.

Citation: Yan J, Wang Y, Shao SH, Mao YF, Li HW, Luo YH. Construction of prokaryotic expression system of 2 148-bp fragment from cagA gene and detection of cagA gene, CagA protein in Helicobacter pylori isolates and its antibody in sera of patients. World J Gastroenterol 2004; 10(8): 1183-1190
URL: https://www.wjgnet.com/1007-9327/full/v10/i8/1183.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i8.1183