Expression patterns of esophageal cancer deregulated genes in C57BL/6J mouse embryogenesis

Table 1 Primers used for semi-quantitative RT-PCR and conditions of PCR reaction

Gene	Dir	Primer sequences (5’-3’)	Product size	Anne (°C)*	PCR cycles
S100A8	F	TGA CAA TGC CGT CTG AAC TG	271	60.4	28
	R	TCC TTG TGG CTG TCT TTG TG
S100A9	F	CAG CAT AAC CAC CAT CAT CG	310	60.1	28
	R	TTA CTT CCC ACA GCC TTT GC
EMP-1	F	AGT GCA AGC CTT CAT GAT CC	599	60.0	28
	R	TCC GAT CTG GGT CTC CAT AC
CSTB	F	GTC CCA GCT TGA ATC GAA AG	208	59.9	28
	R	ATC GTG CCT TTC TTT GTT GG
GAPDH	F	ACCACAGTCCATGCCATCAC	452	According to target gene
	R	TCCACCACCCTGTTGCTGTA

^{*F, forward; R, reverse; Dir, direction; Anne, annealing temperature.}