NOD2 3020insC frameshift mutation is not associated with inflammatory bowel disease in Chinese patients of Han nationality

Figure 1 Identification of frameshiftNOD2 mutation in Chinese population by allele specific PCR. Multiplex PCR was used to generate a nonspecific 533-bp and allele-specific amplified products, a 319-bp fragment (wild type) and a 214-bp fragment (C-insertion type). Lane 1: pBR322 DNA/MspImarkers; Lane 2: Heterozygous for 3020insC; Lane 3: Wild-type control; Lane 4: Homozygote for 3020insC. Numbers on the left are the lengths of the fragments.