Viral Hepatitis
Copyright ©The Author(s) 2004.
World J Gastroenterol. Mar 15, 2004; 10(6): 847-851
Published online Mar 15, 2004. doi: 10.3748/wjg.v10.i6.847
Table 1 Oligonucleotides used to amplify the NS3 region*
Primer sequencesStrandNucleotide positionin HCV genome
First round primers (5’-3’)CCCCATCAC(A/G)TACTC(C/T)ACCTA+4 197
ACA(C/T)GT(A/G)TT(G/A)CAGTC(T/G)ATCAC_4 681
Second round primers (5’-3’)CGAGGATCCGTCCT(T/G)GACCAAGC(A/G)GAGAC+4 315
GCAACTGCAGCT(G/A)G(T/A)(C/T)GG(G/T)ATGAC(A/G)GACAC_4 597