Effects of epidermal growth factor and its signal transduction inhibitors on apoptosis in human colorectal cancer cells

Figure 2 Apoptotic cells stained by annexin V-FITC binding (green) after incubation of SW480 cells with 0. 6 mL/L dimethyl sulfoxide (DMSO, the control group) (A), 225 ng/mL (37.5 nmol/L) EGF in 0.6 mL/L DMSO (B), 225 ng/mL EGF + 2.5 μg/mL (17 nmol/L) EGF antibody in 0.6 mL/L DMSO (C), or 225 ng/mL EGF + 215 ng/mL (0.8 μmol/L) tyrphostin 51 in 0.6 mL/L DMSO serum-free medium (D) for 12 h. MicrograpHmagnified by × 100 is the representative of seven independent experiments (n = 7).