Effects of epidermal growth factor and its signal transduction inhibitors on apoptosis in human colorectal cancer cells

Figure 1 Levels of EGF in medium after incubation of SW480 cells with 0. 06% dimethyl sulfoxide (DMSO, the control group), 225 ng/mL (37.5 nmol/L) EGF in 0.6 mL/L DMSO, 225 ng/mL EGF + 2.5 μg/mL (17 nmol/L) EGF antibody in 0.6 mL/L DMSO, or 225 ng/mL EGF + 215 ng/mL (0.8 μmol/L) tyrphostin 51 in 0.6 mL/L DMSO serum-free medium for 48 h. Data are expressed as mean ± SD (n = 7). ^aP < 0.05 significantly different between the control and EGF-treated groups.