Genotypes of Helicobacter pylori in patients with peptic ulcer bleeding

Table 1 Oligonucleotide primers used for cagA, vacA and iceA genotyping

Region detected	Primer designation	Primer sequence	Size of PCR product (bp)	References
s1 and s2	VA1-F	5’ATGGAAATACAACAAACACACC3’	259	21
	VA1-R	5’CTGCTTGAATGCGCCAAACTTTATC3’	286
s1a	SS1-F	5’GTCAGCATCACACCGCAAC3’	190	9
s1b	SS3-F	5’AGCGCCATACCGCAAGAG3’	187	9
s1c	S1C-F	5’CTYGCTTTAGTRGGGYTA3’	213	17
m1	VA3-F	5’GGTCAAAATGCGGTCATGG3’	290	9
	VA3-R	5’CCATTGGTACCTGTAGAAAC3’
m1T	m1T-F	5’GGTCAAAATGCGGTCATGG3’	290	21
	m1T-R	5’CTCTTAGTGCCTAAAGAAACA3’
m2	VA4-F	5’GGAGCCCCAGGAAACATTG3’	352	9
	VA4-R	5’CATAACTAGCGCCTTGCAC3’
iceA1	iceA1F	5’GTGTTTTTAACCAAAGTATC3’	247	16
	iceA1R	5’CTATAGCCASTYTCTTTGCA3’
iceA2	iceA2F	5’GTTGGGTATATCACAATTTAT3’	229	16
	iceA2R	5’TTRCCCTATTTTCTAGTAGGT3’
lcagA	lcagAD008	5’ATAATGCTAAATTAGACAACTTGAGCGA3’	297	5
	lcagAR008	5 ’TTAGAATAATCAACAAACATCACGCCAT3’