Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or down-regulated in human primary hepatocellular carcinoma

Table 1 Primer sequences and PCR conditions used for synthesis of amplicons applied as probes in Northern blot and RT-PCR

Genes name	Primer (5’-3’)	PCR fragment size (bp)	Annealing temperature	Number of cycles
Pir51	F GTGGAAGATGATGTTGGTGGTG	527	58	32
	R AAGGCGGAGACTCTGATTGG
RbAp48	F GAACTGCCTTTCTTTCAATC	826	58	30
	R ATGGCTCAGACACCTACCTC
Beclin 1	F CTTACCACAGCCCAGGCGAAAC	814	58	30
	R GCCAGAGCATGGAGCAGCAA
Aldolase b	F GCCACTCTCAACCTCAATGC	423	58	32
	R TCTCCTTCCCAACCTACCAC
β-actin	F TGACGGGGTCACCCACACTGTGCC	666	60	25
	R CTTAGAAGCATTGCGGTGGACGATG