In vivo suppressive effect of nuclear factor-κB inhibitor on neutrophilic inflammation of grafts after orthotopic liver transplantation in rats

Figure 1 Effect of PDTC on NF-κB (A), response of inflammatory mediators (B-D), systemic inflammatory recipients (E-F), intrahepatic neutrophil accumulation (G), Mac-1 expression (H), and serum ALT level (I) in OLT rats. Results are the mean ± SD of six rats for each period in each group. A: Intrahepatic p65/relA subunit levels were significantly inhibited in the PDTC cold-preserved group compared with the control group (P < 0.05). P65/relA subunit was significantly different in three preserved groups (P < 0.05). Intrahepatic inflammatory mediator levels were significantly inhibited in the PDTC cold-preserved group compared with the control group (P < 0.05). B: TNF-α was significantly different in three-preserved groups within 0.5 h of reperfusion (P < 0.05), the maximal level reached at 1 h after reperfusion; C: CINC was significantly different in three preserved groups within 2 h after reperfusion (P < 0.05), the maximal level reached at 4 h after reperfusion; D: ICAM-1 was significantly different in three-preserved groups within 0.5 h of reperfusion (P < 0.05), the maximal level reached at 4 h after reperfusion. E: Serum TNF-α levels significantly inhibited in the PDTC cold-preserved group, compared with the control group since 0.5 h after reperfusion (P < 0.05). There is no significantly different in PDTC 40 and PDTC 60 groups (P < 0.05). F: Serum CINC levels significantly inhibited in the PDTC cold-preserved group, compared with the control group since 2 h after reperfusion (P < 0.05). Serum CINC was signifi-cantly different in three-preserved groups (P < 0.05). G: Intrahepatic neutrophil accumulation was significantly inhibited in the PDTC cold-preserved group, compared with the control group (P < 0.05). Intrahepatic neutrophil accumulation was significantly different in three-preserved groups (P < 0.05). H: Mac-1 expression in circulating neutrophil was significantly inhibited in the PDTC cold-preserved group, compared with the control group at 2 h after reperfusion (P < 0.05). There was no great different between Mac-1 expression in PDTC40 and PDTC60 (P > 0.05). I: Serum ALT was significantly inhibited in the PDTC cold-preserved group, compared with the control group (P < 0.05); which was significantly different in three-preserved groups (P < 0.05).