High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris

Figure 2 Integration of SCoVN gene into the P. pastoris GS115 confirmed by PCR using 5’AOX1 primer and 3’AOX1 primer (lane 1: DNA ladder, lane 2: PCR product of pPIC3.5K, lane 3: PCR product of pPIC3.5K-ScoVN, lane 4: PCR product of P.pastoris GS115 without transformation, lane 5: PCR product of control strains, lanes 6 and 7: PCR product of positive recombinants).