High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris

doi:10.3748/wjg.v10.i24.3602

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Dec 15, 2004 (publication date) through Feb 18, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 15, 2004; 10(24): 3602-3607
Published online Dec 15, 2004. doi: 10.3748/wjg.v10.i24.3602

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Figure 1 Construct of expression plasmid pPIC3. 5K-SCoVN under the control of AOX1 promoter (5’ AOX1: promoter fragment; 3’ AOX1 (TT): transcription termination, HIS4 ORF: a select marker, SCoVN was inserted between 5’ AOX1 and 3’ AOX1 (TT), Kanamycin: multi-copy select marker).

Citation: Liu RS, Yang KY, Lin J, Lin YW, Zhang ZH, Zhang J, Xia NS. High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris. World J Gastroenterol 2004; 10(24): 3602-3607
URL: https://www.wjgnet.com/1007-9327/full/v10/i24/3602.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i24.3602