Microarray-based method for detecting methylation changes of p16Ink4a gene 5’-CpG islands in gastric carcinomas

Figure 1 Schematic outline for analysis of DNA methylation based on oligonucleotide microarray[20]. Genomic DNA was bisulfite treated and amplified by PCR for a specific CpG island region of interest. The amplified product was labeled with Cy3 fluorescence dye and hybridized to oligonucleotide probes at-tached to a glass surface. At left an oligonucleotide probe was designed to form a perfect match with a target DNA containing the unmethylated allele. At right a probe was designed to form a perfect match with the methylated DNA target.