Co-mutation of p53, K-ras genes and accumulation of p53 protein and its correlation to clinicopathological features in rectal cancer

Table 1 Sequences of primers used in the study

Gene	Extron/codon	Sequences of primers	Product size
p53	Exons5-6	5’TGTTCACTTGTGCCCTGACT3’
		5’GGAGGGCCACTGACAACCA3’	489 bp
	Exon 7	5’GGCGACAGAGCGAGATTCCA3’
		5’GGGTCAGCGGCAAGCAGAGG3’	286 bp
	Exon 8	5’GACAAGGGTGGTTGGGAGTAGATG3’
		5’GCAAGGAAAGGTGATAAAAGTGAA3’	320 bp
K-ras	Codon 12-13	5’TCAAAGAATGGTCCTGCACC3’
		5’GCCTGCTGAAAATGACTGAA3’	178 bp