Copyright
©The Author(s) 2004.
World J Gastroenterol. Dec 15, 2004; 10(24): 3553-3558
Published online Dec 15, 2004. doi: 10.3748/wjg.v10.i24.3553
Published online Dec 15, 2004. doi: 10.3748/wjg.v10.i24.3553
Table 2 MSP primers used for amplification of p16 gene CpG island. MS and US represent methylated and unmethylated sense primers, respectively. MA1 and MA2, UA1 and UA2 represent methylated and unmethylated antisense primers, respectively
| Primer sets | Sequences (5’→3’) | Size bp | Annealing temp (°C) |
| MS | TTATTAGAGGGTGGGGCGGATCGC | 234 | 65 |
| MA1 | CCACCTAAATCGACCTCCGACCG | ||
| US | TTATTAGAGGGTGGGGTGGATTGT | 234 | 60 |
| UA1 | CCACCTAAATCAACCTCCAACCA | ||
| MS | TTATTAGAGGGTGGGGCGGATCGC | 150 | 65 |
| MA2 | GACCCCGAACCGCGACCGTAA | ||
| US | TTATTAGAGGGTGGGGTGGATTGT | 151 | 60 |
| UA2 | CAACCCCAAACCACAACCATAA |
- Citation: Hou P, Shen JY, Ji MJ, He NY, Lu ZH. Microarray-based method for detecting methylation changes of p16Ink4a gene 5’-CpG islands in gastric carcinomas. World J Gastroenterol 2004; 10(24): 3553-3558
- URL: https://www.wjgnet.com/1007-9327/full/v10/i24/3553.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i24.3553