Microarray-based method for detecting methylation changes of p16Ink4a gene 5’-CpG islands in gastric carcinomas

Table 1 Nucleotide sequences of methylated and unmethylated probes analyzed in oligonucleotide microarray

p16 CpG sites		Oligonucleotide sequences	Tm (°C)
#4-6	M:	5’-NH2-(T)10-CAACCGCCGAACGCAC-3’	56
	U:	5’-NH2-(T)10-CAACCACCAAACACAC-3’	50
#8-10	M:	5’-NH2-(T)10-CAACCGCCGAACGCAC-3’	61
	U:	5’-NH2-(T)10-CCACCACCCACTACCTA-3’	53
#11-13	M:	5’-NH2-(T)10-CCGCCGCCGACTCCAT-3’	61
	U:	5’-NH2-(T)10-CCACCACCAACTCCAT-3’	53
#15-17	M:	5’-NH2-(T)10-AACCGCGACCGTAACCAA-3’	58
	U:	5’-NH2-(T)10-AACCACAACCATAACCAA-3’	50
#18,19	M:	5’-NH2-(T)10-TCTACCCGACCCCGAACC-3’	60
	U:	5’-NH2-(T)10-TCTACCCAACCCCAAACC-3’	55
#20,21	M:	5’-NH2-(T)10-AACAACGCCCGCACCTC-3’	57
	U:	5’-NH2-(T)10-AACAACACCCACACCTC-3’	50
#22,23	M:	5’-NH2-(T)10-ACAACGCCCCCGCCTC-3’	59
	U:	5’-NH2-(T)10-ACAACACCCCCACCTC-3’	52
#24,25	M:	5’-NH2-(T)10-AACTATTCGATACGTTAAAC-3’	48
	U:	5’-NH2-(T)10-AACTATTCAATACATTAAAC-3’	39
#26-28	M:	5’-NH2-(T)10-ATCGACCTCCGACCGTAAC-3’	55
	U:	5’-NH2-(T)10-ATCAACCTCCAACCATAAC-3’	49