Inhibitory effect of heparin-derived oligosaccharides on secretion of interleukin-4 and interleukin-5 from human peripheral blood T lymphocytes

Figure 1 Size separation of heparin oligosaccharides. A: hep-arin degraded with hydrogen peroxide; B: heparin degraded with β -eliminative cleavage; C: heparin degraded with nitrous acids. The reaction products were separated on a Superdex 30 size exclusion column (26 mm×1 200 mm) at a flow rate of 0.5 mL/min in 0.25 mol/L ammonium bicarbonate. Elution profiles were monitored by carbazole assay.