Methylation profile of the promoter CpG islands of 14 “drug-resistance” genes in hepatocellular carcinoma

Figure 3 Methylation profiles of the promoter CpG islands of 24 genes in HCC. Both electrophoretic patterns of the representative PCR products of each of 14 targets and the sequencing verification of one representative PCR product were presented. To indicate the methylation status, the sequenced data are aligned with the wild-type sequence. *, size markers, the bands of 250 bp and 100 bp were shown. U, unmethylated; and M, the hypermethylated. Panels: 1, ABCG2; 2, ATF2; 3, B2M; 4, CAT; 5, CFTR; 6, DCK; 7,GSTpi; 8, OCLN; 9, RAF1; 10,RALBP1; 11, SKP2; 12, SPF45; 13, TOP2B; and 14, TP53.