Methylation profile of the promoter CpG islands of 14 “drug-resistance” genes in hepatocellular carcinoma

Figure 2 MSP testing with the M. Sss I treated normal liver tissue DNA. The in vitro methylated DNA from the normal liver tissue was used for MSP along with the untreated. Panels: 1, ABCG2; 2, ATF2; 3, B2M; 4, DCK; 5, RAF1; 6, RALBP1; 7, SKP2; 8, SPF45; 9, TOP2b; and 10, TP53. The PCR conditions for OCLN gene were assessed by PCR in three liver cancer cell lines, 7721, 7402, and Hep3B (panel 11). U, unmethylated allele; M, methylated allele, NL, normal liver tissue; *the DNA size marker: DL-2000.