Real-time determination of human telomerase reverse transcriptase mRNA in gastric cancer

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 10, Issue 23

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

All Articles published online

Item

Count

PDF

346

HTML

2195

Figures (1-2)

210

Tables (1-2)

199

Sum=2950

Dec 1, 2004 (publication date) through Nov 24, 2024

Times Cited of This Article

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Brief Reports

World J Gastroenterol. Dec 1, 2004; 10(23): 3514-3517
Published online Dec 1, 2004. doi: 10.3748/wjg.v10.i23.3514

Table 1 Sequences of primers and probes used in this study

Gene and oligonucleotide	Sequence	PCR product size (bp)
GAPDH
Upper primer	5’-GAAGGTGAAGGTCGGAGTC-3’
Lower primer	5’-GAAGATGGTGATGGGATTTC-3’	226
Probe	5’-(FAM) CAAGCTTCCCGTTCTCAGCC (TAMRA)-3’
hTERT
Upper primer	5’-TGACACCTCACCTCACCCAC-3’
Lower primer	5’-CACTGTCTTCCGCAAGTTCAC-3’	95
Probe	5’-(FAM) ACCCTGGTCCGAGGTGTGTCCCTGA (TAMRA)-3’

The primers placed in different exons, were tested to ensure that they did not amplify the contaminated genomic DNA.

Citation: Hu LH, Chen FH, Li YR, Wang L. Real-time determination of human telomerase reverse transcriptase mRNA in gastric cancer. World J Gastroenterol 2004; 10(23): 3514-3517
URL: https://www.wjgnet.com/1007-9327/full/v10/i23/3514.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i23.3514