Methylation profile of the promoter CpG islands of 14 “drug-resistance” genes in hepatocellular carcinoma

Table 3 The primers for MSP analysis

GenBank No.	Gene symbol		Sense 5’-3’	Antisense 5’-3’	Position	Size(bp)
NT_016354	ABCG2	m	TGTCGCGTTGAGTCGTTA	AACGTCCCCGATACTTCG	-698 to -464	235
		U	TGTGTTTTGTTGTGTTGAGTTGT	TCACTCTAATTCATTCCATTCAATC	-591 to -457	135
NT_005403	ATF2	m	GGGTCGGAATAACGAACG	ATCACCTCGAATACTCCTAACG	230 to 375	146
		U	GGAGGGGTTGGAATAATGAAT	CCCATTTCCCATCACCTCAA	220 to 379	160
NT_010194	B2M	m	ATTTGGTATTGCGTCGTTG	ACGAAACGAAACATCTCGAC	-128 to 23	151
		U	TTTTTAATTTGGTATTGTGTTGTTG	AACTCACACTAAATAACCTCCAAAC	-134 to 83	217
NT_009237	CAT	m	AGTAGCGGGTCGCGTAG	AACCACCCGAACCTATCG	118 to 372	255
		U	GGAAGGAGTAGTGGGTTGTGT	AACCACCCAAACCTATCACA	112 to 372	261
NT_007933	CFTR	m	AGAGGTCGCGATTGTCGTT	CGACTTTCTCCACCCACTACG	-316 to -114	203
		U	TTAAAGAGAGGTTGTGATTGTTGTT	TCCTTCACTCCCTCACCA	-322 to -174	149
NT_006216	DCK	m	TATACGCGCGGTTTCGT	CGCCGACGAATATCGAA	-168 to 27	195
		U	TTTTTTGTTATATGTGTGGTTTTGT	TACCCCTCAACCCTCACC	-176 to -75	102
NT_078018	OCLN	m	TGCGTTCGTTAGGTGAGC	CGAATCCCAACTCGAAAACG	538 to 753	216
		U	GTTAGGTGTGTTTGTTAGGTGAGT	CACACCTCTCTAATTCCCACA	532 to 772	241
NT_022517	RAF1	m	TCGGTCGTTTTGGAAGTC	CCCTAAAACGCGAAACG	-72 to 180	252
		U	GGTTTGGTTGTTTTGGAAGTT	CACCAAATATAACCACCTCCCACT	-2 to 183	185
NT_010859	RALBP1	m	GGGTAAGTCGTTCGTTTTCG	CCTCTCCGCTCAAACGACT	-495 to -215	281
		U	GTTAGTATTATATTGGGGTAAGTTGTTTG	CCCTTCATCCCCAAACTCA	-510 to -371	140
NT_006576	SKP2	m	GTCGTAGCGTCGTTCGTT	CTACAACCCGCTCTACTTCG	-198 to 10	208
		U	TTTTTTAGTTAGTTGTAGTGTTGTTTGTT	ACCCACTCTACTTCACAACCAC	-209 to 5	214
NT_077569	SPF45	m	AGTGTCGTTCGGTTTCGTT	CCTCGAAAACTCCGACTACG	-216 to -6	211
		U	GTGGAGTGTTGTTTGGTTTTGT	AACTTACATCTAACACCTCCCAAA	-220 to -67	154
NT_022517	TOP2B	m	TGGGTTTCGTCGTTTCGT	CCGCGCTAAACCCGAAC	-241 to -66	176
		U	GGTTGTTGGGTTTTGTTGTTTT	TTCTCCTCAACCACCACACTAA	-254 to -60	195
NT_010718	TP53	m	CGGAGTCGAGAGTTCGTG	CCGAAAACACTTTACGTTCG	157 to 279	123
		U	GTTGAAAATATATGGAGTTGAGAGTTT	CTTTCCACAACAATAACACACTTC	190 to 291	102
NT_033903	GSTP1	m	GCGATTTCGGGGATTTTA	ACGACGACGAAACTCCAA	-183 to 16	199
		U	GTTGGGGATTTGGGAAAG	TATAAAAATAATCCCACCCCACT	-230 to -28	203