Expression of Dnmt1, demethylase, MeCP2 and methylation of tumor-related genes in human gastric cancer

Table 2 Primer sequences and PCR conditions for RT-PCR, MSP and sequencing

Primers	Sense (5’→3’)	Antisense (5’→3’)	Product size (bp) PCR program	GenBank accession number
β -Actin	GGA GTC CTG TGG CAT CCA CG	CTA GAA GCA TTT GCG GTG GA	322 bp 94 °C 30 s, 60 °C 1 min, 72 °C 1 min, 27 cycles	BC023204
p16INK4A RT-PCR	CCC GCT TTC GTA GTT TTC AT	TTA TTT GAG CTT TGG TTC TG	355 bp 94 °C 1 min, 58 °C 1 min, 72 °C, 1 min, 35 cycles	L27211
c-myc RT-PCR	CCA ACA GGA GCT ATG ACC TC	CTC GGT CAC CAT CTC CAG CT	290 bp 94 °C 1 min, 52 °C 1 min, 72 °C, 1 min, 35 cycles	V00568
MeCP2 RT-PCR	ACT CCT CAG AAT ACA CCT TGC TT	TGA GGC CCT GGA GGT CCT	112 bp 95 °C 1 min, 50 °C 1 min, 72 °C, 1 min, 35 cycles	MCB, 2000; 20: 3316
mbd2 RT-PCR	AAC CCT GCT GTT TGC TTA AC	CGT ACT TGC TGT ACT CGC TCT TC	101 bp 94 °C 1 min, 60 °C 1 min, 72 °C, 1 min, 40 cycles	AF072242
p16INK4A M-MSP	TTA TTA GAG GGT GGG GCG GAT CGC	GAC CCC GAA CCG CGA CCG TAA	150 bp 95 °C 30 s, 65 °C 30 s, 72 °C, 1 min, 40 cycles	X94154
p16INK4A UM-MSP	TTA TTA GAG GGT GGG GTG GAT TGT	CAA CCC CAA ACC ACA ACC ATA A	151 bp 95 °C 30 s, 60 °C 30 s, 72 °C, 1 min, 40 cycles	X94154
c-myc M-MSP	TAG AAT TGG ATC GGG GTA AA	CGA CCG AAA ATC AAC GCG AAT	131 bp 95 °C 30 s, 56 °C 30 s, 72 °C, 1 min, 40 cycles	AF002859
c-myc UM-MSP	TAG AAT TGG ATT GGG GTA AA	CCA ACC AAA AAT CAA CAT GAA T	132 bp 95 °C 30 s, 56 °C 30 s, 72 °C, 1 min, 40 cycles	AF002859
hMSH2 sequencing 1st round	TGT TTA GAA AGA AAA AGG GA	AAA CCT CCT CAC CTC CT	94 °C 1 min, 55 °C 1 min, 72 °C, 1 min, 35 cycles	AB006445
hMSH2 sequencing 2nd round	AAA TAT TGG GAG GAG GAG GA	ACC CAC TAA ACT ATT TCC CA	327 bp 94 °C1 min,, 55 °C 1 min, 72 °C, 1 min, 35 cycles	AB006445