Nuclear factor-kB p65 (RelA) transcription factor is constitutively activated in human colorectal carcinoma tissue

Figure 2 Electrophoretic mobility shift assay demonstrating increased nuclear translocation and DNA binding of NF-kB. A: lane 1, positive control (using Hela nuclear extract); lane 2, normal; lanes 3-6, adenocarcinoma; lanes 7-10, adenoma. B: lane 1, positive control (using Hela nuclear extract); lanes 2-3, specific competitor (using excess of unlabeled oligonucleotide); lanes 4-5, adenoma; lanes 6-7, adenocarcinoma; lane 4 and 6, supershift (addition of p65 antibodies to the nuclear extracts).