Replication and gene expression of mutant hepatitis B virus in a transgenic mouse containing the complete viral genome with mutant s gene

Figure 1 Southern blot analysis of 20 μg of total DNA ex-tracted from transgenic mouse tail tissues of lineages G145R-15 and G145R-18. Lane 0: Positive control; Lane 1: Lineage G145R-15; Lane 2: Lineage G145R-15; Lane 3: F₁ transgenic mice of lineage G145R-15; Lane 4: F₁ transgenic mice of lineage G145R-18; Lane 5: Lineage of transgenic mice harbouring pro-totype HBV genome; Lane 6: Non-transgenic mice as negative control. All DNA samples were RNase treated before gel electrophoresis. The filters were hybridized with a digoxigeninlabeled HBV specific DNA probe.