A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons

Figure 3 RFLP of S amplicon of HBV from 187 nested PCR-positive serum samples from Chinese carriers. Lane M: 100 bp ladder from 100-600 bp fragments, P: Undigested PCR product. A: After the first step of parallel digestion with BsrI and StyI, the samples were divided into four groups: Genotypes B, C, A/E/G and D/F/H; B: Groups A/E/G and D/F/H could be further resolved by the second parallel digestion. In this study, genotypes E, F, G and H were not observed, while genotypes B and C were the major ones.