A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons

doi:10.3748/wjg.v10.i21.3132

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Nov 1, 2004 (publication date) through Nov 12, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Nov 1, 2004; 10(21): 3132-3136
Published online Nov 1, 2004. doi: 10.3748/wjg.v10.i21.3132

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Figure 2 Diagrammatic representation of the position of PCR primers and RFLP analyses for HBV genotyping. The second round PCR products, which were 585 bp, were digested by (1) BsrI, (2) StyI, (3) DpnI, (4) HpaII and (5) EaeI. Genotypes B and C could be typed in one step using parallel digestion with BsrI and StyI.

Citation: Zeng GB, Wen SJ, Wang ZH, Yan L, Sun J, Hou JL. A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons. World J Gastroenterol 2004; 10(21): 3132-3136
URL: https://www.wjgnet.com/1007-9327/full/v10/i21/3132.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i21.3132