A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons

Figure 1 Genotype-specific sites recognized by restriction enzymes. GeneBank accession numbers and consensus sequences from HBV genotypes A to H are listed. The shaded letters indicate the sequences recognized by the relevant enzyme. StyI recognizes sequence C|C (A/T) (A/T) GG; BsrI, C|CAGT; DpnI, GA|TC; HpaII, C|CGG; EaeI, (C/T) |GGCC (A/G). *nt position number is from the unique EcoRI site according to the reference sequence × 001587 (genotype C).