Yeast expression and DNA immunization of hepatitis B virus S gene with second-loop deletion of α determinant region

Figure 3 Screening integrants and SDS-PAGE analysis. A: Screening integrants using PCR with AOX1 primers. Lane M2: DNA marker; Lane 1: PCR products of Mut⁺ transformant without gene of interest; Lane 2: PCR products of Mut^s transformants with pHBY; Lane 3: PCR products of Mut^s transformants with pHB-SDY; Lane 4: PCR products of pPIC9 alone. Lane M1 were DNA markers. B: Coomassie-stained SDS-PAGE analysis of the recombinant proteins of pHB-SDY and pHBY. Lane M3: protein marker; Lane 5: cell lysate super-natants of yeast alone; Lane 6: yeast transformed with pPIC9; Lane 7: yeast transformed with pHBY; Lane 8: yeast trans-formed with pHB-SDY.