Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells

Figure 1 Transient expression of E2-661 in mammalian cells. A: Schematic representation of plasmids encoding E2-661. HBV preS1 (21-47) tag and HCV E2-661 (384-661) signal peptide are indicated. Numbers refer to amino acids of the HCV polyprotein. B: Western blot analysis of E2-661 expressed in mammalian cells with a rabbit polyclonal anti-E2 antibody RE2116. Lanes on the left of all blots were transfected with the empty vector while those on the right were transfected with pSecTagB/sE2. 1-2, BHK-21; 3-4, HeLa; 5-6, Huh7 and 7-8, HepG2. E2-661 was indi-cated by arrowhead.