Construction of exogenous multiple epitopes of helper T lymphocytes and DNA immunization of its chimeric plasmid with HBV pre-S2/S gene

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Oct 15, 2004 (publication date) through Nov 4, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Oct 15, 2004; 10(20): 2979-2983
Published online Oct 15, 2004. doi: 10.3748/wjg.v10.i20.2979

Table 1 Primers and oligonucleotide fragments used in mte5 splicing and construction of eukaryotic expression plasmids

	Sequences (5’→3’)
F1	ATGGCTAAAA CCATCGCCTA TGATGAAGAA GCTCGTCGTG GTCTGGAACG TGGTCTGAAT GCCTCCGAT
F2	GTCCAGGCAG CAACGAATTT AGCTCCCTGC AGCCAGTGTC CAGGCAGAAC ATCGGAGGCA TTCAGACCA
F3	AATTCGTTGC TGCCTGGACC CTGAAAGCTG CCGCTGGAAG ACACGTTGTT ATCGATAAGA GCTTCGGAA
F4	TTCGGTGATT CCGATAAACT TGGAATTAGC TTTGATGTAC TGCTGAGGGC TTCCAAGGCT CTTATCGAT M ` T E -
MTE-P1	TCGGATCCCA TGGCTAAAAC CATCGCC
MTE-P2	GCTCTAGACT TTCGGTGATT CCGATAA
HBV-P1	CCTAAGCTTA TGCAGTGGAA CTCCACT
HBV-P2	TGGAATTCCT TAAATGTATA CCCAGAG
Bam-1	TCGGATCCCA TGGCTAAAAC CATCGCC
Bam-2	GCGGATCCCT TTCGGTGATT CCGATAA
Hinc-1	TCGTTGACAA TGGCTAAAAC CATCGCC
Hinc-2	GCGTTGACAA TTCGGTGATT CCGATAA
Xba-1	TCTCTAGACA TGGCTAAAAC CATCGCC
Xba-2	GCTCTAGACT TTCGGTGATT CCGATAA
Acc-1	TCGTATACAT GGCTAAAACC ATCGCCT
Acc-2	CGCGTATACT TCGGTGATTC CGATAAA

Citation: Gao WJ, Peng XM, Xie DY, Xie QF, Gao ZL, Yao JL. Construction of exogenous multiple epitopes of helper T lymphocytes and DNA immunization of its chimeric plasmid with HBV pre-S2/S gene. World J Gastroenterol 2004; 10(20): 2979-2983
URL: https://www.wjgnet.com/1007-9327/full/v10/i20/2979.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i20.2979