Stable expression of human cytochrome P450 2D6*10 in HepG2 cells

Figure 3 Representative chromatograms of metabolites in supernatant. 10 μL of supernatant was injected into a Water HPLC equipped with a Shimadzu RF-535 fluorescence detector. A CLC phenyl column (15 cm×4.5-mm i.d.) was used to sepa-rate the metabolites. The mobile phase consisted of a mixture of 30% acetonitrile, 1% acetic acid, and 0.05% triethylamine in water. The flow rate through the column at 25 °C was 0.75 ml·min^-1. The excitation and emission wavelengths of the fluorescence de-tector were 285 nm and 310 nm, respectively. The retention times for dextrophan and dextromethorphan were 6.5 min and 16.8 min, respectively. The retention time of an unidentified metabolite was 8.9 min.