Heparanase mRNA expression and point mutation in hepatocellular carcinoma

doi:10.3748/wjg.v10.i19.2795

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Oct 1, 2004 (publication date) through Dec 2, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 1, 2004; 10(19): 2795-2799
Published online Oct 1, 2004. doi: 10.3748/wjg.v10.i19.2795

Table 3 Sequencing and corresponding series number

Number	Nucleotide sequence						Remarks
	TTGG	GCCCGACGTC	GCATGCTCCC	GGCCGCCATG	GCGGCCGCGG	GAATTCGATT	Plasmid DNA
409-468	TTCGATCCCA	AGAAGGAATC	AA¹CCTTTGAA	GAGAGAAGTT	ACTGGCAATC	TCAAGTCAAC	HPA cDNA
469-528	CAGGATATTT	GCAAATATGG	ATCCATCCCT	CCTGATGTGG	AGGAA^3,5AAGTT	ACGGTTGGAA	HPA cDNA
529-588	TGGCCCTACC	AGGAGCAATT	GCTACTCCGA	GAACACTACC	AGAAAAAGTT	CAAGAACAGC	HPA cDNA
589-648	ACCTACTCAA	GAAGCTCTGT	AGATGTGCTA	TACACTTTTG	CAAACTGCTC	AGGACTGGAC	HPA cDNA
649-708	TTGATCTTTG	GCCTAAATGC	GTTATTAAGA	ACAGCAGATT	TGCAGTGGAA	CAGTTCTAAT	HPA cDNA
709-768	GCTCAGTTGC	TCCTGGACTA	CTGCTCTTCC	AAGGGGTATA	ACATTTCTTG	GGAACTAGGC	HPA cDNA
769-828	AATGAACCTA	ACAGTTTCCT	TAAGAAGGCT	GATATTTTCA	TCAATGGGTC	GCAGTTAGGA	HPA cDNA
829-888	GAAGATTTTA	TTCAATTGCA	TAAACTTCTA	AGAAAGTCCA	CCTTCAAAAG⁴	T⁶GCAAAACTC	HPA cDNA
889-948	TATGGTCCTG	ATGTTGGTCA	GCCTCGAAGA	AAGACGGCTA	AGATGCTGAA	GAGCTTCCTG	HPA cDNA
949-993	AAGGCTGGTG	GAGAAGTGAT	TGATTCAGTT	ACATGGCATC	ACTAC²		HPA cDNA
	AATCACTAGT	GAATTCGCGG	CCGCCTGCAG	GTCGACCATA	TGGGAGAGCT	CCCAACGCGT	Plasmid DNA
	TGAATCACTA	GTGAATTCGC	GGCCGCCTGC	AGGTCGACCA	TATGGGAGAG	CTCCCAACGC	Plasmid DNA
	GTTG						Plasmid DNA

^1,2The segments with italics and boldface corresponded to the primers used in RT-PCR. ^3,4The letters represented the mutated bases. ^5,6The parts with underline represented the codons.

Citation: Chen XP, Liu YB, Rui J, Peng SY, Peng CH, Zhou ZY, Shi LH, Shen HW, Xu B. Heparanase mRNA expression and point mutation in hepatocellular carcinoma. World J Gastroenterol 2004; 10(19): 2795-2799
URL: https://www.wjgnet.com/1007-9327/full/v10/i19/2795.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i19.2795