Copyright
©The Author(s) 2004.
World J Gastroenterol. Sep 15, 2004; 10(18): 2719-2722
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2719
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2719
Figure 2 Typical elution profiles of DHPLC analysis and sequencing.
Panel A: DHPLC elution profiles for RIZ(A)9 tract, column temperature: 56 °C, flow rate: 0.9 mL/min, mobile phase: 0.1 mol/L TEAA (pH7.0), linear gradient: 51%-60% B in 4.5 min, detected with UV at 260 nm; Panel B: Sequence tracings for the same samples. The upper panel is a normal control, and the lower is a frameshift mutation (1-bp deletion). The arrow indicates the mutant nucleotide; Panel C: DHPLC elution profiles for pro704 LOH, column temperature: 50 °C, flow rate: 0.75 mL/min, mobile phase: 0.1 mol/L TEAA (pH7.0), linear gradient: 49%-55% B in 7 min, detected with UV at 260 nm.
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Citation: Pan KF, Lu YY, Liu WG, Zhang L, You WC. Detection of frameshift mutations of
RIZ in gastric cancers with microsatellite instability. World J Gastroenterol 2004; 10(18): 2719-2722 - URL: https://www.wjgnet.com/1007-9327/full/v10/i18/2719.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i18.2719