Construction of prokaryotic expression system of ltB-ureB fusion gene and identification of the recombinant protein immunity and adjuvanticity

doi:10.3748/wjg.v10.i18.2675

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Sep 15, 2004 (publication date) through Jul 28, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 15, 2004; 10(18): 2675-2679
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2675

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Figure 3 rLTB-UreB expression induced with different dos-ages of IPTG. Lane 1: Protein marker (Shanghai Shisheng); Lane 2: Blank control; Lane 3: Non-induced with IPTG; Lanes 4-6: Induced with 0.1, 0.5 and 1.0 mmol/L IPTG, respectively; Lanes 7 and 8: Bacterial precipitate and supernatant induced with 0.5 mmol/L IPTG, respectively.

Citation: Yan J, Wang Y, Shao SH, Mao YF, Li HW, Luo YH. Construction of prokaryotic expression system of ltB-ureB fusion gene and identification of the recombinant protein immunity and adjuvanticity. World J Gastroenterol 2004; 10(18): 2675-2679
URL: https://www.wjgnet.com/1007-9327/full/v10/i18/2675.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i18.2675