Polymerase synthesis and potential interference of a small-interfering RNA targeting hPim-2

Figure 4 Inhibitory effects of siRNA on mRNA level of hPim-2. A: Electrophoresis of RT-PCR products of hPim-2 gene and β-actin gene in colon cancer cells transfected with siRNA I,II,III. B: Quantitation of inhibitory percentage of hPim-2 mRNA in transfected cells. Each level of PCR product of hPim-2 gene was. quantitated and normalized to the level of β-actin. Inhibitory rate was calculated by comparing to the control cells. The results were expressed as means ± SD from independent experiments. P < 0.05 vs the cells transfected with lipofectamine alone.